Colonic Crypt‐Derived 3D Gut Organoid from Hypertensive Rat Maintain In vivo Characteristics

Substantial evidence links gut dysbiosis to hypertension in both animal models and in patients with high blood pressure. Increased blood pressure and hypertension-associated pathologies in normotensive animals transplanted with fecal microbiota from hypertensive animals, support the concept of microbiota-gut communication as an important early signal initiating and establishing hypertension. We hypothesized that colonic cryp-3D organoids would retain the characteristics of the in vivo gut epithelium form which they were derived, and would provide a model system to investigate epithelial-microbiota communication in hypertension.